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Fractionation Formula Pdf Free Download Pdf
determination of the experimental conditions leading to the best fractionation efficiency is achieved by carrying out preliminary experiments. a good starting point is to consider the ability of the sample to flow through the channel with the solvent of the eluent. the retentive behavior is measured as the volume of the sample that remains in the channel, normalized to the volume of the sample before entering the channel. this parameter is referred to as the retention efficiency (re) and can be related to the fractionation efficiency by means of the following equation [ 45 ].
the main applications of af4 are the separation of nanoparticles for medical applications (drug delivery, cancer imaging, imaging agents, etc.) and the separation of inorganic nanoparticles (e.g. quantum dots, magnetic nanoparticles, carbon nanotubes, nanoemulsions, etc.). since the aim of this review is to assess the role of af4 in the field of nanomedicine, we will focus on the latter of these two areas. nanoparticles are generally isolated in aqueous solutions, either as suspensions or as formulations. the separation of such particles can be performed either by size, by density, or by both. in the case of size-based separation, the sample is injected in a flow of eluent and the different fractions are collected and analyzed. in af4, the size-based separation is performed based on the ability of the particles to flow through a continuous flow of the eluent. in this case, the separation is defined not only by the particle size but also by its shape, so that the eluent is a function of the geometry of the channel. the term microfluidics is used to define the field of micro and nano-scale devices fabricated with polymers, metals, ceramics, or other materials and devoted to the manipulation of fluids, in particular for medical applications.
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